The first club dock, for the convenience of yachting members, was constructed in 1929 as a gift from Alfred I. duPont. After 20 years, it became necessary to build a new dock, which was made possible through the enthusiasm of many members, foremost among whom was Leon T. Cheek.

In addition to developing its golf course, the Club soon grew into a center of many other social and sporting activities. The Clubhouse has been the scene of many happy and distinguished social events. In 1933, two clay tennis courts made a brief appearance on the lower riverfront terrace, but were shortly abandoned. Although a swimming pool had been proposed from the earliest days of the Club, it was not until after World War II that one was built for and enjoyment of the members and their families. 

The mid-30s were critical years at Timuquana as the Depression was taking its toll on private country clubs across the country. At one time Timuquana's membership dropped to 51 and the Club was operated without a manager. By 1936, it became obvious that a thorough reorganization of the financial structure of the Club was imperative. Through able and patient guidance, the necessary readjustments were accomplished, and the Club has successfully operated ever since under the sound arrangements established at that time.

By the 1950s, it became apparent that the Club had outgrown the original Clubhouse and that a major project had to be undertaken to provide adequate facilities for the membership. After several years of careful study by many boards and committees, a Permanent Improvement Committee presented a comprehensive plan for rebuilding and remodeling the old building, in addition to making substantial additions. The project was approved at a special meeting of the Founder members in the winter of 1957 and work commenced the following June. Throughout the remainder of the year, the membership patiently endured many inconveniences while construction deprived them of the use of a Clubhouse. Upon completion, the new traditionally Southern-style Clubhouse was opened with a festive two-day reception, beginning December 16, 1958.

 In 1963, the Club added a new swimming pool, four tennis courts, several service buildings and a new men's lounge.

 In the 1970s a young pro named Bobby Duval came to Timuquana. Among his best pupils was son David (pictured left, with his father, circa 1980), who grew up playing golf at the Club.

By the early 1980s, David became a top youth golfer nationally. He was the U.S. Junior Amateur Champion in 1989 and led Episcopal High School of Jacksonville to a state championship. He then went on to become a four-time All-American at Georgia Tech and was the NCAA National Player of the Year in 1993. David spent two years on the Nike Tour and earned his PGA Tour card in 1995. Throughout the late 1990s, he saw great success on the PGA Tour and rose to become the number one ranked professional player in the world by 1999. David won the British Open Championship in 2001 and to-date has 13 victories on the Tour. All Timuquanans remember with pride the March weekend in 1999 when David won at THE PLAYERS Championship in Ponte Vedra Beach, the very same day father Bobby won his first Champions Tours event, the Emerald Coast Classic, in Pensacola. 

In 1994, a golf course committee was formed to study the current golf course and its usage. Along with professional consultants and direction from Robert L. Weed, this special course advisory committee designed a plan to renovate the golf course. The Club's members approved the plan in December 1995, and construction began in April of 1996. The project was completed in October of the same year, and the new course opened.

Part of the golf course renovation was a historic partnership with the U.S. Navy in which the Club negotiated with the Navy at NAS Jax to include an effluent water program to irrigate the Club's golf course. Final connections were made in the summer of 1997 and the Club began accepting the effluent water in the fall of the same year.

Timuquana celebrated its 75th anniversary in 1998 with a week-long extravaganza. The annual Founders’ Day Golf Tournament kicked-off the celebration, followed by the Annual Meeting and a formal dinner dance with New York's Alex Donner Orchestra entertaining a sellout crowd of more than 450. Weekend events included a family carnival on the waterfront lawn, a repeat of the 1927 costume golf tournament, the dedication of a new flagpole next to the St. Johns River and fireworks marking the event's grand finale.

1998 was also a year for Club improvements as the Long Range Planning Committee convened to discuss renovation plans for the north end of the Club. The plans called for enlarging the Pow Wow Room and adding a Cocktail Lounge, Fitness Center and a new floating dock system.

In September 2002, Timuquana hosted the Senior Amateur Championship of the United States Golf Association. After lengthy preparations by club committees and staff, the championship was completed with outstanding success. From the Timuquana Navy that brought our guests from the hotel downtown to the evening dinner to the final putt by champion Greg Reynolds on a beautiful golf course, compliments and expressions of delight were received.

In 2007, the Board of Governors and the Long Range Planning Committee initiated a study to improve the Club’s facilities. Successive Boards and Long Range Planning Committees conducted studies and developed and refined a plan for a Club Improvement program, which culminated in the overwhelming approval of a program at a special meeting of the Club’s Founder and Honorary Life members held on September 28, 2010.

Demolition and reconstruction of the Clubhouse, together with a refurbishment of the Living Room, River Room and other areas began on March 28th, 2011, and was substantially completed on December 14, 2011. The golf course was closed from May 2, 2011 until October 13, 2011, to redo the greens and bunkers, re-grass the fairways and tees, lengthen a few tees and re-work the 11th green.

New pool restrooms were built in early 2012.